cheap jerseys Wholesale Jerseys Wholesale Jerseys nfl jerseys china wholesale nfl jerseys cheap nhl jerseys wholesale nhl jerseys cheap nba jerseys cheap mlb jerseys wholesale mlb jerseys cheap nike jerseys cheap nike jerseys free shipping cheap authentic nfl nike jerseys nfl football jerseys china cheap authentic football jerseys replica football jerseys cheap football jerseys authentic cheap football jerseys paypal cheap nfl football jerseys wholesale football jerseys cheap china replica football jerseys china nfl jerseys supply cheap replica jerseys china replica football jerseys china cheap jerseys Wholesale Jerseys China wholesale authentic jerseys nfl jerseys from china cheap nfl jerseys china buy cheap nfl jerseys wholesale nfl jerseys china cheap nhl jerseys china wholesale nhl jerseys china cheap nba jerseys china wholesale nba jerseys china cheap mlb jerseys china wholesale mlb jerseys china nike jerseys china cheap nike jerseys with free shipping nfl nike jerseys wholesale cheap custom football jerseys replica nfl football jerseys cheap authentic football jerseys china football jerseys replica replica football jerseys cheap wholesale nfl jerseys supply replica nfl jerseys nike replica nfl jerseys cheap jerseys for sale Wholesale Jerseys From China wholesale sports jerseys nfl jerseys free shipping cheap nfl jerseys from china where to buy cheap nfl jerseys wholesale nfl jerseys from china cheap nhl jerseys from china wholesale nhl jerseys from china cheap mlb jerseys from china wholesale mlb jerseys from china nike jerseys free shipping cheap nike jerseys china discount nfl nike jerseys cheap custom football jerseys cheap football jerseys for sale wholesale football jerseys usa wholesale football jerseys china buy football jerseys from china football jerseys from china wholesale cheap jerseys supply cheap replica sports jerseys replica jerseys nfl wholesale cheap jerseys wholesale jerseys from china free shipping wholesale jerseys supply cheap nfl jerseys wholesale cheap nfl jerseys paypal wholesale nfl jerseys paypal cheap nhl jerseys wholesale nhl hockey jerseys wholesale cheap nba basketball jerseys wholesale basketball jerseys cheap mlb jerseys paypal wholesale mlb jerseys paypal wholesale nike jerseys paypal wholesale authentic nike jerseys wholesale nfl nike jerseys china custom college football jerseys where to buy football jerseys cheap youth replica football jerseys cheap football jerseys kids cheap football jerseys nike authentic football jerseys wholesale nike football jerseys wholesale cheap blank football jerseys cheap nfl jerseys supply replica jerseys china wholesale replica jerseys cheap replica nfl football jerseys cheap nfl jerseys free shipping where to get cheap nfl jerseys wholesale nhl jerseys free shipping cheap nba jerseys free shipping wholesale nba jerseys cheap cheap mlb jerseys free shippping wholesale mlb jerseys free shipping wholesale nike jerseys cheap nike jerseys wholesale nfl nike jerseys china custom youth football jerseys cheap wholesale football jerseys cheap football jerseys for women wholesale cheap football jerseys football jerseys cheap authentic nfl football jerseys china wholesale jerseys supply wholesale replica jerseys cheap replica throwback jerseys football replica jerseys cheap authentic jerseys Wholesale Nike NFL Jerseys nfl jerseys supply cheap nfl jerseys online cheap nfl nike jerseys wholesale nfl jerseys cheap cheap nhl jerseys online wholesale nhl jerseys paypal cheap stitched nba jerseys wholesale nba basketball jerseys wholesale mlb jerseys usa wholesale nike jerseys china cheap wholesale nfl nike jerseys wholesale nike football jerseys cheap throwback football jerseys wholesale kids football jerseys wholesale football jerseys suppliers authentic football jerseys for cheap wholesale ladies football jerseys cheap football jerseys with free shipping supply cheap jerseys reviews cheap youth replica jerseys nfl jerseys replica cheap nfl jerseys usa cheap nfl jerseys from china wholesale authentic nfl jerseys cheap nhl jerseys wholesale hockey jerseys cheap nba authentic jerseys authentic nba jerseys wholesale cheap mlb replica jerseys wholesale mlb baseball jerseys nike jerseys cheap jerseys china chinese nfl nike jerseys wholesale blank football jerseys cheap throwback football jerseys for sale cheap football jerseys stitched wholesale football jerseys youth wholesale nfl nike football jerseys womens football jerseys wholesale supply jerseys cheap kids replica jerseys replica sports jerseys wholesale cheap jerseys canada wholesale jerseys usa Wholesale Jerseys From China nfl jerseys china wholesale cheap nfl authentic jerseys cheap nfl jerseys wholesale wholesale nike nfl jerseys cheap nhl jerseys canada china wholesale nhl jerseys cheap retro nba jerseys buy cheap jerseys online nba cheap custom mlb jerseys wholesale baseball jerseys nike jerseys wholesale chinese wholesale nike jerseys cheap nfl nike jerseys china blank football jerseys wholesale wholesale nfl football jerseys wholesale nfl football jerseys from china wholesale football jerseys from china wholesale youth nfl football jerseys wholesale nike nfl football jerseys china football jerseys wholesale wholesale jerseys supply reviews wholesale jerseys replica wholesale nike jerseys paypal cheap nfl nike jerseys from china cheap youth practice football jerseys cheap wholesale jerseys discount nfl football jerseys wholesale nike elite football jerseys wholesale replica football jerseys cheap stitched football jerseys wholesale nfl jerseys supply review wholesale replica jerseys china cheap nfl replica jerseys replica nfl jerseys China Wholesale Jerseys wholesale authentic football jerseys cheap jerseys paypal cheap ncaa jerseys paypal cheap mlb jerseys paypal chinese wholesale jerseys nike football jerseys china cheap nike jerseys usa Wholesale Jerseys China nfl football jerseys discount cheap football jerseys online nfl jerseys cheap china replica nfl jerseys wholesale china nike jerseys cheap football jerseys cheap replica nfl jerseys nfl replica jerseys china wholesale jerseys usa cheap replica football jerseys replica nfl jerseys china Wholesale Jerseys From China football jerseys wholesale china youth football jerseys wholesale nfl jerseys china wholesale nike jerseys wholesale cheap china nfl nike jerseys cheap nfl authentic jerseys wholesale nhl hockey jerseys cheap nba jerseys for sale cheap nfl jerseys wholesale wholesale jerseys nhl cheap nba jerseys online wholesale nike nfl jerseys cheap wholesale nfl jerseys where to buy cheap nhl jerseys cheap nhl jerseys canada cheap stitched nfl jerseys cheap nike nfl jerseys china wholesale nhl jerseys buy wholesale jerseys nfl jerseys china cheap cheap retro nba jerseys cheap replica jerseys cheap wholesale jerseys from china buy cheap jerseys online nba cheap youth football jerseys cheap fake football jerseys cheap custom mlb jerseys nfl wholesale nike jerseys cheap custom football jerseys nike jerseys wholesale cheap nba jerseys wholesale cheap authentic nba jerseys cheap jerseys from china buy cheap jerseys from china cheap nike nfl jerseys cheap sports jerseys Wholesale Jerseys China buy wholesale jerseys wholesale authentic jerseys nfl jerseys from china cheap nfl jerseys china cheap nfl jerseys usa cheap nike nfl jerseys nfl jerseys from china nfl jerseys supply nfl jerseys cheap china authentic nfl jerseys wholesale custom nfl jerseys cheap customized nfl jerseys cheap china nfl jerseys cheap cheap official nfl jerseys super bowl jerseys nike super bowl jerseys buy super bowl jerseys wholesale super bowl XLIX jerseys super bowl 49 jerseys wholesale super bowl jerseys sale super bowl jerseys from china 2015 super bowl jerseys form china where to buy super bowl jerseys cheap nfl draft jerseys nfl super bowl jerseys nike super bowl jerseys super bowl 49 jerseys cheap super bowl jersey super bowl 49 jerseys wholesale nfl super bowl XLIX jerseys super bowl 49 jerseys patch 2015 super bowl jerseys form china nike nfl draft jerseys wholesale blank jerseys cheap blank football jerseys wholesale wholesale youth football practice jerseys cheap jerseys authentic reviews supply cheap jerseys reviews cheap stitched jerseys for kids wholesale replica jerseys china wholesale jerseys for women cheap jerseys online free shipping buy wholesale jerseys china nike jerseys cheap cheap sports jerseys and hats wholesale jerseys and hats cheap kids jerseys china cheap sports jerseys china wholesale jerseys china nike wholesale cheap jerseys usa cheap jerseys from china kids cheap jerseys wholesale usa cheap wholesale jerseys authentic super bowl jerseys nike super bowl jerseys buy super bowl jerseys cheap super bowl jerseys super bowl jerseys wholesale super bowl jerseys china cheap nfl super bowl jerseys fake super bowl jerseys where to buy super bowl jerseys cheap nfl draft jerseys cheap nfl jerseys cheap mlb jerseys cheap nfl jerseys paypal wholesale nfl jerseys cheap wholesale nfl jerseys cheap nhl jerseys online cheap hockey jerseys wholesale hockey jerseys cheap stitched nba jerseys cheap nba jerseys wholesale nba jerseys cheap mlb jerseys cheap nike jerseys nfl jerseys from china cheap nfl jerseys supply wholesale nike jerseys nfl jerseys china wholesale wholesale nfl jerseys cheap nhl jerseys cheap nhl jerseys cheap nba jerseys cheap nba jerseys cheap mlb jerseys replica sports jerseys cheap jerseys buy cheap jerseys china cheap jerseys cheap jerseys paypal Wholesale Jerseys wholesale jerseys usa wholesale sports jersey nfl jerseys china cheap nfl jerseys free shipping cheap nfl jerseys buy cheap nfl jerseys wholesale nfl jerseys wholesale authentic nfl jerseys cheap nhl jerseys cheap mlb jerseys wholesale cheap nfl jerseys cheap jerseys from china buy cheap jerseys china cheap jerseys cheap jerseys paypal Wholesale Jerseys wholesale jerseys usa wholesale nba jerseys from china cheap nhl jerseys canada football jerseys for cheap nike super bowl jerseys nhl jerseys china fake oakleys Balenciaga arena high louboutin replica chinese nhl jerseys fake oakley sunglasses Balenciaga black replica louboutin shoes wholesale nhl jerseys fake oakleys White balenciaga shoes replica louboutin wedges cheap nhl jerseys fake oakleys Balenciaga arena suede replica louboutin boots cheap nfl jerseys Balenciaga monochromes cheap red bottom shoes sale cheap super bowl jerseys replica oakley sunglasses balenciaga arena shoe in white replica louboutin shoes wholesale nba jerseys replica louboutin boots fake oakleys Christian louboutin sale nfl jerseys from china cheap louboutin heels fake oakleys Christian louboutin red bottoms wholesale jerseys china nike replica christian louboutin boots fake oakleys Shoes free shipping fake oakley sunglasses Balenciaga mens shoes cheap louboutin pumps cheap nfl nike jerseys fake oakley sunglasses Balenciaga arena sneakers replica louboutin heels cheap nba jerseys china fake oakley sunglasses Red balenciaga sneakers louboutin replica shoes wholesale nba jerseys from china fake oakley sunglasses Balenciaga arena lambskin low Sneakers louboutin replica pumps super bowl jersey shop fake oakley sunglasses stitched jerseys from china cheap red bottom shoes sale cheap oakley sunglasses Louboutin store cheap sports jerseys usa cheap red bottom shoes cheap oakley sunglasses Louboutin shop online cheap stitched nfl jerseys cheap louboutin boots fake oakley sunglasses Mens christian louboutin cheap custom nfl jerseys cheap replica louboutin shoes fake oakley sunglasses Cheap louboutins wholesale pro jerseys replica louboutin heels fake oakley sunglasses Louboutin boutique wholesale nfl jerseys nfl jerseys supply cheap louboutin outlet fake oakleys Christian louboutin mens shoes cheap nfl super bowl jerseys cheap louboutin heels cheap oakley sunglasses Discount louboutin shoes cheap wholesale jerseys authentic cheap louboutin shoes online discount oakley sunglasses giuseppe zanotti for men cheap jerseys from china authentic buy cheap red bottom shoes cheap oakley sunglasses men giuseppe zanotti shoes authentic nfl jerseys wholesale louboutin replica pumps fake oakleys sneakers giuseppe zanotti china nfl jerseys cheap cheap louboutin wedges fake oakleys men giuseppe sneakers cheap official nfl jerseys red bottom shoes for cheap cheap oakleys giuseppe zanotti for men replica oakley sunglasses cheap nike jerseys replica louboutin heels giuseppe zanotti shoe nhl jerseys china cheap louboutin pumps oakley sunglasses discount giuseppe zanotti high tops nfl jerseys from china cheap red bottom shoes sale oakley sunglasses discount shop designer shoes online chinese nhl jerseys cheap louboutin shoes online cheap oakleys giuseppe shoes men wholesale nhl jerseys china cheap louboutin outlet white maison martin margiela margiela high tops wholesale nike jerseys buy cheap nhl jerseys martin margiela sneaker super bowl jerseys china margiela e boutique cheap nfl jerseys louis vuitton mens sneakers cheap mlb jerseys Louis Vuitton trainers cheap jerseys gucci shoes Wholesale Jerseys gucci leather shoes cheap nhl jerseys wholesale soccer jerseys nfl jerseys supply cheap christian louboutin high heels men giuseppe zanotti
QUẢNG CÁO
 
Truy cập
Đang online: 1
Tổng số: 513,795
CHI TIẾT
Phân lập và xây dựng quy trình định lượng một số hợp chất hướng tác dụng chống oxy hoá của cây móng bò leo (Bauhinia bracteata Benth. Fabaceae)
Isolation of natural compounds for antioxidant activities from Bauhinia bracteata Benth. Fabaceae
Nguyễn Thị Thu Hạnh, Nguyễn Đức Tuấn
Số: 15 - Tháng 2/2021 - Trang 26-34

Chi Bauhinia đã được biết có một lịch sử lâu dài về tác dụng điều trị các bệnh cấp tính và mạn tính như kháng khuẩn, kháng viêm, giảm đau, sỏi mật, hạ đường huyết, … trong y học dân gian và y học cổ truyền. Trong thời gian gần đây trên thế giới đã có một số công trình nghiên cứu thành phần hóa học và hoạt tính sinh học như chống oxy hóa, kháng khuẩn … từ vỏ, hạt, lá, hoa và vỏ thân của nhiều loài trong chi này như B. variegata, B. purpurea, B. vahlii, B. forficata. Chi Bauhinia có khoảng 300 loài, ở Việt Nam có khoảng 33 loài trong đó Bauhinia bracteata Benth. gặp nhiều ở Đăk Lăk, người dân nơi đây còn gọi là móng bò leo hay móng bò rừng, thường dùng lá để hãm nước uống hàng ngày theo kinh nghiệm dân gian nhằm bảo vệ sức khỏe, chống bệnh tật hay trị sỏi mật, hạn chế sỏi tái phát … Tuy đã có công bố về phát hiện loài này trên thế giới và được mô tả về đặc điểm sinh thái, phân bố, phân loại thực vật trong nhiều sách về thực vật tại Việt Nam của nhiều tác giả như Võ Văn Chi, Phạm Hoàng Hộ; nhưng cho đến nay, chưa có tác giả nào công bố kết quả nghiên cứu cụ thể về Bauhinia bracteata Benth. ở trong nước cũng như trên thế giới. Xuất phát từ những lý do trên, nghiên cứu này công bố các kết quả đầu tiên về phân lập, xác định cấu trúc các hợp chất từ dịch chiết ethyl acetat của lá cây móng bò leo và tác dụng chống oxy hóa so với acid ascorbic nhằm chứng minh giá trị y học về tác dụng chống oxy hóa của loài B. bracteataso với các loài khác trong chi đã được biết đến; Xây dựng quy trình định lượng một số hợp chất này bằng phương pháp HPLC góp phần làm phong phú thêm kho tàng tri thức về cây thuốc và đa dạng của nguồn thảo dược hướng tác dụng chống oxy hóa tại Việt Nam.

Nguyên liệu

Lá móng bò leo thu hái vào tháng 3/2019 tại phường Thành Nhất, thành phố Buôn Ma Thuột, tỉnh Đắk Lắk.

Phương pháp nghiên cứu

- Khảo sát thực vật và phân tích ADN

- Sàng lọc hoạt tính chống oxy hóa

- Phương pháp chiết xuất thường quy

- Xây dựng quy trình định lượng các hợp chất phân lập

- Thẩm định quy trình phân tích

Chuẩn bị mẫu

- Dung môi pha mẫu

- Dung dịch đối chiếu gốc acid gallic

- Dung dịch đối chiếu gốc hyperin

- Dung dịch đối chiếu

- Dung dịch thử

Kết luận

Đã phân lập được 4 hợp chất từ cao ethyl acetat của lá móng bò leo đạt độ tinh khiết sắc ký trên 96% và có hoạt tính chống oxy hóa cao hơn so với chất đối chiếu acid ascorbic cũng như xây dựng và thẩm định quy trình định lượng đồng thời acid gallic và hyperin trong lá móng bò leo bằng phương pháp HPLC-PDA đạt yêu cầu theo hướng dẫn của ICH và AOAC. Những kết quả nghiên cứu này góp phần vào việc bổ sung cơ sở dữ liệu hóa học và tác dụng sinh học của cây móng bò leo, giúp định hướng cho những nghiên cứu tiếp theo về thành phần hóa học cũng như các tác dụng sinh học của cây móng bò leo.

Bauhinia bracteata Benth. Fabaceae is a species that grows a lot in Dak Lak province, and belongs to the Bauhinia genus. This genus contains many common species in Viet Nam that have the main chemical components such as flavonoid, tannin, organic acid,… with important bioactivities such as antioxidants, antibacterials, anti-inflammatories, anti-hyperglycemias, and anti-diarrheas, … So far, there have been no published studies on phytochemicals and bioactivities of Bauhina bracteata. Therefore, this study was conducted with the aim of contributing to determine chemical composition, and demonstrate antioxidant activity of Bauhina bracteata. Leaves of Bauhinia bracteata were collected in March 2019 at Thanh Nhat ward, Buon Ma Thuot City, Dak Lak province. The morphological characteristics and microsurgery of leaves, and ADN analysis of Bauhinia bracteata were determined. The total and insoluble ash, and humidity were 8.18%, 0.40%, and 9.08%, respectively. The content of extractable substances in 80% alcohol was 15.15%. The main chemical components in Bauhinia bracteata leaves were flavonoid, tannin, organic acid, saponin,… By using exhausted extraction and evaporation methods and combining with liquid – liquid partition extraction, the n-hexane (18 g), chloroform (40 g), and ethyl acetate (120 g) extracts were obtained from 6 kg of Bauhinia bracteata leaves. Among of them, ethyl acetate extract showed the highest antioxidant activity. Ethyl gallate (46 mg, chromatographic purity over 98%), gallic acid (540 mg, purity over 97%), hyperin (148 mg, purity over 98%), and myricitrin (38 mg, purity over 96%) were isolated from 60 g of ethyl acetate extract. All isolated compounds had lower IC50 than ascorbic acid. Gallic acid showed the lowest (2.93 µg/ml), thus the strongest antioxidant activity and 2.95 times stronger than ascorbic acid. Ethyl gallate and myricitrin, and hyperin showed 2.73, 2.36 times stronger than ascorbic acid, respectively. The HPLC-PDA method for simultaneous determination of gallic acid and hyperin was developed and validated, using mixture of methanol and 0.1% phosphoric acid pH 3.5 (40:60) as mobile phase in isocratic mode, and InertSustain C18 column (250 x 4.6 mm; 5 µm). Validation results showed that the method was suitable for the HPLC system, selective, wide linearity range, low detection and quantitation limits, high accurate and precise, and conformed the requirements for a quantitative determination process. The results of this study contributed to phytochemical and bioactivity database of Bauhinia bracteata, and refer to further studies on chemical composition as well as biological activities of this plant.

Quay trở lại

Bản quyền thuộc về Tạp chí Dược học – Bộ Y tế

Địa chỉ: 138 A Giảng Võ - Quận Ba Đình - Thành phố Hà Nội

Điện thoại: 04.37367717 – 04.37368367 - E-mail: tapchiduocbyt@gmail.com